Adult periodontal disease is associated with anaerobic gram-negative bacteria, in particular Prophyromonas gingivalis, Treponema denticola, and Tannerella forsythensis. Recent studies have linked the onset of periodontal disease in young adults with wisdom teeth or third molars. Wisdom teeth are difficult to clean due to their location and are prone to periodontal infection which can spread to other teeth. This study investigated the distribution of the three pathogens in wisdom teeth and in anterior teeth of Asian Indian patients at a private dental clinic. The BANA (N-benzoyl-DL-arginine-2-napthyamide) enzyme assay was performed on subgingival plaque samples taken during routine scaling. DNA was extracted from paper points used by the dentist to collect samples of subgingival fluid. The polymerase chain reaction (PCR) detected specific pathogens based on the amplification of signature sequences of the small subunit16S rRNA genes. We examined the relationship between bacterial distribution, BANA score, demographic factors (age and gender) and clinical parameters (pocket depth, dental history and bleeding on probing).